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Team:Peking/HumanPractice/ModeliGEM
From 2013.igem.org
Practical Analysis
In order to test if our toolkit works practically, we took water samples from our beloved Weiming Lake. Is she pure as she appears? Or is she polluted by aromatic compounds? Weiming Lake is a famous scenic spot in Peking University. In Chinese, "Weiming" means "without being named". Because many people in history tried to name it by capturing its essence, but all failed, so a famous scholar gave the lake this name.
Located in the center of the campus, the lake was once the royal garden in Qing Dynasty. Today it is still regarded as a sacred lake by many visitors. On the hill of the southern part of the Weiming Lake, there is a pagoda named Boya. It is an imitation of ancient tower. The combination of the lake and the tower forms a distinctive landscape. Students in Peking University can be seen reading and studying beside the lake. Thus，testing water sample from this lake is meaningful for us.
Water from Weiming Lake in Peking University was sampled at 15:30 and 19:30, sterilized by 0.22 um filter. LB solution was prepared by using each of the water samples. Antibiotics were added into two tubes containing the two water samples to prepare 50 ml engineered bacteria’s medium. After 12 hours cultivation according to the inducing process, OD 600 and F515 were tested. Then, aromatic compounds was calculated by dose-effect curve.
The aromatic compounds concentration was parallelly measured three times by microplate reader. The induction ratios are as follow. According to the table, the water samples didn’t induce the manipulated bacteria obviously and each kind of aromatic compounds was below the detection limit. According to the experiment, Weiming Lake’s water sample didn’t induce manipulated bacteria obviously. It can be drawn that benzoic acid, aminosalicylic acid and phenylog derivatives concentration of Weiming Lake are all under the water pollution standard of our country (GB8978-1996). The conclusion are drawn as follow, water from Weiming Lake is not natural water, but running water from taps, which was purified by complex procedures. It is anticipatable that the water from Weiming Lake is up to standard and there is no aromatic compounds within.
To see our test protocols,please
