Team:DTU-Denmark/Methods/PCR
From 2013.igem.org
Generel example on a normal PCR-program
- 98°C for 2:00
- 98°C for 0:10
- 59°C for 0:30
- 72°C for 2:00
- Go to number 2 - repeat 35
- 72°C for 5:00
- End
Always use heated lid and set the machine to wait until the lid have the right temperature. If not default set to lid temperature to 104°C.