Team:Chiba/Project/Future

Future

Tasks we couldn’t finish until Asia contest
    ・We couldn’t double knock down fieF and fur with CRISPRi yet, but we are confident that we can knock down any gene by CRISPRi.
    ・In the not so distant future, we would break E. coli’s iron homeostasis using CRISPERi and would uptake more iron into E. coli.
    ・Even if E. coli’s iron homeostasis would break, over expression of human ferritin could save E. coli alive.

Tasks that didn’t go like we wanted
    ・knocking out gor or trxB didn’t lead to a grate change of redox potential like TCO89 in yeast.
    ・It might be hard to oxidize iron in E. coli strain SHuffle®(It isn’t oxidized enough). But by over expression of strong oxidase, maybe we can oxidize iron inside E. coli, make magnetite and magnetize E. coli.

A Use of magnetized E. coli
    ・E. coli would proliferates infinitely if we feed them and give them iron. So if we use it to a magnetic recording media, perhaps the storage capacity could increase infinitely.
    ・Magnetized E. coli could be attracted by magnetic force, so we can separate magnetized E. coli easily from the colony of E. coli.
    ・Combining with some BioBrick (i.e.University of California Berkley 2006, Addressable Conjugation in Bacterial Networks), we can make a magnetic switch. Also, in combination with quorum sensing, we can make a magnetic switch. Magnetic switch has good points like we wrote in introduction.