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Team:DTU-Denmark/Notebook/27 June 2013
From 2013.igem.org
Contents |
208 lab
Main purposes today
Run gels to verify yesterdays PCR-prodcuts. Make new PCR to amplify the signal peptides and the backbone.
who were in the lab
Gosia, Henrike, Kristian
Procedure
Signal peptides on 4% gels 80V for 45 min. All other PCR-products on a 1% gel 100V for 45 min. Two new PCRs with ramp from 70°C → 60°C and 60°C → 50°C. Sec signal peptide on 70°C → 60°C and TAT signal peptide with new primer set 2 and 3 respectively on program 60°C → 50°C.
