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Contents 1 Community Outreach 1.1 Biosecurity Background 1.2 Project 1.3 References

Community Outreach

A key principle of iGEM is education and outreach to the general public about synthetic biology. We invited a local paper -"The Daily Texan"- and a television crew from "TsTV" for a laboratory tour and were featured on the [http://www.youtube.com/watch?v=1Fvnnl4ZInA local news] and in a [http://www.dailytexanonline.com/news/2013/04/22/ut-students-work-on-entry-for-genetic-engineering-competition press release] detailing our daily activities in the lab. The team also sponsored a [http://www.dailytexanonline.com/2013/09/18/fbi-workshop-raises-awareness-against-biosecurity-threats FBI biosecurity workshop] attended by scientists, government officials, law enforcement, and members of the community. We fostered an environment of collaboration and transparency by bringing a diverse group of people together to discuss measures we can implement as a local community to ensure that synthetic biology is not used for harmful purposes. Finally, we presented last year's research on caffeine addicted bacteria at the popular science education event "Hot Science-Cool Talks".

Biosecurity Background

With the growth and advancement of synthetic biology, bioterrorism has become a greater concern. Currently, the government does not mandate that DNA synthesizing companies conduct precautionary screening measures to ensure that [http://en.wikipedia.org/wiki/Select_agent select agent] genes are not unknowingly produced and sold. Instead, the government only provides screening guidelines for potentially harmful oligonucleotides and are not enforced.[9]

The Select Agents and Toxins list is composed of harmful bacteria, viruses, and toxins that the Department of Health and Safety is most concerned may be used as weapons for bioterrorism. [4] Various oligo screening methods have been developed such as [http://guidance.tau.ac.il/overview.html Guidance], Top Homology, and Best Match. Best Match, though less fool proof than other screening algorithms, like Top Homology, is the more popular screening method chosen by synthesizing companies due to it being much more efficient and compartmentalized. When an oligo is ordered, the sequence is screened for homology to any unique sequence of the Select Agents and Toxins. Best Match screens 200 base pairs at a time and screens using different reading frames. If the oligo is more homologous to a Select Agent or Toxin than any other sequence found in international sequence reference databanks, such as NCBI, the order is flagged. The owner of the account must disclose their purpose for ordering such an oligo and must be granted permission to have a dangerous oligo synthesized.

IGSC (International Gene Synthesis Consortium) companies screen ordered oligos as six different reading frames. The amino acid sequences are screened for protein similarity in a Regulated Pathogen Database. The Regulated Pathogen Database is currently being modified and will be a collaboration of the Select Agent list, the Australian Group list, and other regulated pathogens set by various countries. [10]

There are various inadequacies with Best Match. Best Match is not able to detect unrelated accounts conspiring to order harmful oligos. The Select Agents and Toxins list does not account for every harmful agent and toxin. Thus, harmful oligos that are not listed can be synthesized because they are not screened for. Forbidden oligos are distributed to government, university, non-profit, and industry researchers according to IGSC which may be a lenient list with access to these oligos. [10]

Project

In this project, screening for the Ebola virus was imitated using a program written in the Python language. The program generates random sequences with 200 base pairs in length and BLAST searches for them in the NCBI database to test for homology. The program also extracts random sequences from NCBI and screens them for homology to Ebola in 200 base pair segments testing each reading frame. The theoretical ordered sequences are only screened for the unique sequences that Ebola has to avoid false positives.

Ebola virus encodes seven polypeptides from its RNA genome of ca. 19.0 kb, including the glycoprotein (GP), nucleoprotein (NP), RNA-dependent RNA polymerase (L), VP35, VP30, VP40, and VP24. [1]

References

1. Han, Z., Boshra, H., Sunyer, J. O., Zwiers, S. H., Paragas, J., Harty, R. N., Han, Z., et al. (2003). Biochemical and Functional Characterization of the Ebola Virus VP24 Protein : Implications for a Role in Virus Assembly and Budding Biochemical and Functional Characterization of the Ebola Virus VP24 Protein : Implications for a Role in Virus Assembly and Budding. doi:10.1128/JVI.77.3.1793
2. Bucher, J. R. (2009). ACTION :, 74(227), 38–46.
3. Framework, S. (2011). correspondence Strengths and limitations of the federal guidance on synthetic DNA, 29(3), 1–3. doi:10.1038/nbt0311-208
4. HHS AND USDA SELECT AGENTS AND TOXINS 7 CFR Part 331 , 9 CFR Part 121 , and 42 CFR Part 73. (n.d.)., 331.
5. No Title. (n.d.)., 45–49.
6. Preventing the misuse of gene synthesis Commercialized GM crops and yield. (2009)., 27(9), 800–801.
7. Schmidt, M., & Giersch, G. (2011). Dna s s, 1–15.
8. Select, F., & Program, A. (n.d.). Restricted Experiment Guidance Document, (Cdc).
9. Federal Register/Vol. 74, No. 227/Friday, November 27, 2009/Notices
10. IGSC-Harmonized-Screening-Protocol