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Overview

Model of Recombinase Expression

Model of the Switch

Model of Inducible Hbif

Introduction

switch modeling

• goals: produce both states of switch, use model to optimize switch

mass action modeling

• what is mass action

• pros of mass action modeling, i.e. why we used it

• form of mass action models/solvability doc

analytical modeling:

• benefits

   • naturally standardized
   
   • given parameters, it precisely predicts behavior

   • accurately predicts the data needed to determine parameters

   • allows avoidance of numerical errors like rounding

• ERSESCO

   • equation

   • reduction

   • solution

   • equilibration

   • stabilization

   • calibration

   • optimization

Model of Recombinase Expression

Model of the Switch

Model of Inducible Hbif

Expression Model

Equation:

Reduction: None

Solution:

Equilibration:

Stabilization:

Calibration:

Optimization:

SimBiology:

Expression Model Derivation

Switch Model

Equation:

Reduction:

Solution:

Equilibration:

Stabilization:

Calibration:

Optimization:

SimBiology:

Switch Model Derivation

Inducible Hbif Model

Equation:

Reduction:

Solution:

Equilibration:

Stabilization:

Calibration:

Optimization of Hbif:

Optimization of ON:

SimBiology:

Inducible Hbif Model Derivation

Future Directions

Data!