Constructing Parts to Express Active Human Insulin

INSULIN

The sequence we used for insulin was obtained from NCBI. It is the coding sequence for human insulin. The sequence obtained from NCBI was codon optimized for use in P. pastoris utilizing IDT codon optimization software. Expressing and obtaining pure has insulin from Pichia cultures will reduce allergic responses, inconsistent purity and concentration, and host rejection in patients who take insulin as a treatment of type I and type II diabetes.

PCSK1

PCSK1 encodes for preprotein convertase TYPE I, which is regarded as the most important enzyme in the first step of insulin processing in humans. Since our model organism P. pastoris lacks this enzyme it is hypothesized that its addition will increase over the lineage.

Goal
To construct BioBrick parts that contain the human insulin and PCSK1 genes, codon optimized for P. pastoris.

Background

Methods

Parts List

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