From 2013.igem.org

208 lab

Main purposes today

New PCR with x7-polymerase.

who were in the lab

Kristian

Procedure

Made PCRs on pZA21, GFP SF TAT, GFP SF Sec and RFP all samples where made in duplicates.

In tube 1+2+3+4+5+6 where pZA21. In 7+8+9+10+11+12 GFP SF TAT In 13+14+15+16+17+18 GFP SF Sec In 19+20+21+22+23+24 RFP

First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. Last program was 69°C annealing and 1:00 extension time with tube: 9+10.

Results

The gel from the days PCR. From first lane is a 100 bp ladder and thereafter the well number follows the numbers of the tubes -1. Note that tube number 15 is missing

The gel from the days PCR. From first lane is a 100 bp ladder and thereafter tube 20,21,22,23 and 24. Note that there is also primer blur in subsequent wells to the right; these are just double test wells to test previous PCR products

Conclusion from today

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