Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/5.8 Infect With Phage

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Phage Purification May - June Notebook: Experiments

Phage Purification

March-April

5.8 Infecting With Phage

I) Purpose

Infect liquid bacterial cultures with phage for phage amplification.

II) Expected Outcome

Phage available to purify.

III) Materials Used

Cultures of bacteria to infect

Phage from Lysate (Experiment 5.6)

IV) Actual Procedure

add 100 microL of phage from lysate to bacterial culture. Allow proper infection time before using in purification protocol.

For BL 21: infect with T7 phage for 24 - 48 hours

For W3110: infect with T4 phage for 7-8 hours

Place in 37^◦ C for infection

V) Results

The phage infected the cultures, as proven by visible clearing when compared to the noninfected control cultures of bacteria.