Metabolismt Optimization
We plan to choose the Fatty acid metabolic pathway for our system's application. We combine three key enzymes in Fatty acid metabolic pathway with our light controlled system. They are TesA, FabI and FabZ which affect three very pivotal reaction process. We expect that we can regulate the proportion of three enzymes by three different light-dCas9 systems to control the productive rate of fatty acid. We use the modeling tools to simulate the regulate process to verification our plan.
Circuit
Three main circuit are as follows:
Main Reaction
We call three main reactions which we controlled reaction A, B and C.
Models and Explains
In order to make the explaination of our model short and sweet, we simplify three main reactions as follows:
A: A-CoA + M-CoA --> B-ACP (FabI & FabZ)
B: B-ACP + nM-CoA --> F-ACP (FabI & FabZ)
C: F-ACP --> Fatty acid (TesA)
For reaction A, it is a double substrates enzymatic reaction, consideration of substrate self-inhibition, we set this reaction model:
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