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Team:DTU-Denmark/Notebook/2 July 2013
From 2013.igem.org
Contents |
208 lab
Main purposes today
Create dilution series for biolector experiment.
Weighed:
- 2.3187g Ammonium acetate
- 0.2557g Sodium nitrate
- 0.2080g Sodium nitrite
Diluted each in 10mL M9 medium.
Created dilution series as in https://docs.google.com/spreadsheet/ccc?key=0AkM9Z7voM1otdFZ5SWstOWpURGs2S3M5bElMMllkenc
| Salt | mM | ||
|---|---|---|---|
| 300 | |||
| 30 | |||
| 3 | |||
| 0.3 | |||
| 3000 | |||
| 300 | |||
| 30 | |||
| 3 | |||
| 300 (Master) | |||
| 30 | |||
| 3 | |||
| 0.3 | |||
| 0.03 | 2013-07-02 | yes | no |
Who was in the lab
Henrike, Jakob, Kristian, Gosia
Procedure
PCR for 7 fragments:
Conclusion from today
Inconsistent results from the first PCR where obtained with bands in some of the reactions but not in the duplicates. Even the g-Block was not seen though we have easily amplified that before with very bright bands. Something must have gone wrong and that's why we made the additional 34rxns which will be analyzed on gels tomorrow.
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