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Team:DTU-Denmark/Notebook/2 July 2013
From 2013.igem.org
Contents |
208 lab
Main purposes today
Create dilution series for biolector experiment.
Who was in the lab
Helen, Natalia, Ariadni
Procedure
Weighed:
- 2.3187g Ammonium acetate
- 0.2557g Sodium nitrate
- 0.2080g Sodium nitrite
Diluted each in 10mL M9 medium.
Created dilution series as in https://docs.google.com/spreadsheet/ccc?key=0AkM9Z7voM1otdFZ5SWstOWpURGs2S3M5bElMMllkenc
Each solution was diluted with media.
| Salt | mM |
|---|---|
| Sodium Nitrate | 300 |
| Sodium Nitrate | 30 |
| Sodium Nitrate | 3 |
| Sodium Nitrate | 0.3 |
| Sodium Nitrite | 300 (Master) |
| Sodium Nitrite | 30 |
| Sodium Nitrite | 3 |
| Sodium Nitrite | 0.3 |
| Sodium Nitrite | 0.03 |
| Ammonium Acetate | 3000 |
| Ammonium Acetate | 300 |
| Ammonium Acetate | 30 |
| Ammonium Acetate | 3 |
Conclusion from today
No conclusions yet.
208 lab afternoon
purpose
doing colony pcr
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