Outreach

123

• Figure 4

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  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 4. Negative control: Fluorescence microscopy showing how RFP expession changes with time on addition of 0mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 5

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  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 5. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.05mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 6

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  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 6. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.1mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 7

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  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 7. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.2mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 8

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  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 8. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.4mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 9

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  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 9. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.8mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

345

• Figure 4

  +

  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 4. Negative control: Fluorescence microscopy showing how RFP expession changes with time on addition of 0mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 5

  +
  <

  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 5. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.05mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 6

  +

  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 6. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.1mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 7

  +
  <

  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 7. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.2mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 8

  +

  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 8. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.4mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.

• Figure 9

  +
  <

  10 minutes	30 minutes
  60 minutes	120 minutes
  240 minutes	Figure 9. Fluorescence microscopy showing how RFP expession changes with time on addition of 0.8mM IPTG in B.subtilis containing our HBsu-RFP BioBrick.