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Exeter/14 August 2013
From 2013.igem.org
Revision as of 10:38, 29 September 2013 by Fentwistle (Talk | contribs)
MiniPrep
We Miniprepped the following parts:
- Promoter and RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002])
- Magenta pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012 BBa_K592012])
- Yellow pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592010 BBa_K592010])
- Yellow pigment with RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_S05058 BBa_S05058])
- Terminator ([http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015])
- RFP control ([http://parts.igem.org/wiki/index.php?title=Part:BBa_J04450 BBa_KJ04450])
NanoDrop results
| Part | ng/ul |
|---|---|
| RFP control | 80.7 |
| Yellow w/ RBS | 60.8 |
| Promoter and RBS | 127.8 |
| Magenta pigment | 46.9 |
| Terminator | 89.9 |
| Yellow pigment | 59.0 |
| KAN plasmid | 23.6 |
Digestion
| Part | ng/ul from NanoDrop | ul need to have 250ng DNA | 16ul - required ul DNA (amount of water needed for digestion) |
|---|---|---|---|
| RFP control | 80.7 | 3.10 | 12.9 |
| Yellow w/ RBS | 60.8 | 4.11 | 11.89 |
| Promoter and RBS | 127.8 | 2.0 | 14.0 |
| Magenta pigment | 46.9 | 5.33 | 10.67 |
| Terminator | 89.9 | 2.80 | 13.2 |
| Yellow pigment | 59.0 | 4.24 | 11.76 |
| KAN plasmid | 23.6 | 10.60 | 5.4 |
