From 2013.igem.org
MiniPrep
We Miniprepped the following parts:
- Promoter and RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002])
- Magenta pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012 BBa_K592012])
- Yellow pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592010 BBa_K592010])
- Yellow pigment with RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_S05058 BBa_S05058])
- Terminator ([http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015])
- RFP control ([http://parts.igem.org/wiki/index.php?title=Part:BBa_J04450 BBa_KJ04450])
NanoDrop results
| Part | ng/ul
|
| RFP control | 80.7
|
| Yellow w/ RBS | 60.8
|
| Promoter and RBS | 127.8
|
| Magenta pigment | 46.9
|
| Terminator | 89.9
|
| Yellow pigment | 59.0
|
| KAN plasmid | 23.6
|
Digestion
| Part | ng/ul from NanoDrop | ul need to have 250ng DNA | 16ul - required ul DNA (amount of water needed for digestion)
|
| RFP control | 80.7 | 3.10 | 12.9
|
| Yellow w/ RBS | 60.8 | 4.11 | 11.89
|
| Promoter and RBS | 127.8 | 2.0 | 14.0
|
| Magenta pigment | 46.9 | 5.33 | 10.67
|
| Terminator | 89.9 | 2.80 | 13.2
|
| Yellow pigment | 59.0 | 4.24 | 11.76
|
| KAN plasmid | 23.6 | 10.60 | 5.4
|
Gel of digest
| Lane | Part
|
| 1 | empty (well looked damaged)
|
| 2 | 1kb GeneRuler Ladde
|
| 3 | Terminator
|
| 4 | RFP control
|
| 5 | skipped, looked damaged
|
| 6 | skipped, looked damaged
|
| 7 | Magenta pigment
|
| 8 | KAN plasmid
|
| 9 | Yellow pigment w/ RBS
|
| 10 | Yellow pigment
|
| 11 | 1kb GeneRuler Ladder
|
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