"

From 2013.igem.org

{ "date":"2013-07-01", "author":"viola", "title":"PCR of the double terminator BBa_B0015", "content":"Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities:

	B0015 in pSB1AK3 219,4 ng/ µl	B0015 in pSB1C3 413,3 ng/ µl
H20	33.3 µl	33.3 µl
DNA	0.5 µl	0.5 µl
DNTPs	1 µl	1 µl
pref. FW	2.5 µl	2.5 µl
suff. RV	2.5 µl	2.5 µl
BUFFER	10 µl	10 µl
Phusion pol.	0.5 µl	0.5 µl

}}</html>", "tags":"EFE" }