Team:DTU-Denmark/Timeline

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Timeline

Contents

Project Timeline

January

February

  • 2013-02-25 Project selected: Conversion of ammonia to nitrous oxide in E. coli

March

  • 2013-03-11 Biobrick workshop hosted for KU and SDU
  • 2013-03-13 Project kickoff meeting with supervisors

April

May

June

  • 2013-06-04 Team competency mapping
  • 2013-06-09 Work on wiki started
  • 2013-06-29 Team BBQ

July

Week 27

  • Hello World project complete and successful. From a cytoplasmic extraction, we have observed GFP in the periplasm and RFP in the cytoplasm.
  • Conducted the toxicity experiment and completed data analysis: average ammonia concentrations in wastewater are not toxic to E. coli.

Week 28

  • Designed, ordered and received primers to extract genes from Nitrosomonas and Pseudomonas
  • Began work on cloning genes out of Nitrosomonas europaea and Pseudomonas aeruginosa. All isolations successful so far except for Nir.

Week 29

Week 30

  • Ordered new primers to extract Nir gene in two pieces.
  • Despite successful PCR and transformation of AMO, HAO and cytochromes, we only have viable colonies for the cytochromes. Expect that this is due to the fact that we are expressing periplasmic and membrane proteins, which is causing excessive stress on the cells.
  • Began work on pBAD synthetic promoter library so that we will be able to induce a low level of expression of our periplasmic genes.

Week 31

  • The week no PCR worked -- ran 96 PCRs with varying conditions to debug what is not working
  • Saturday BBQ with KU

August

Week 32

Week 33

Week 34

Week 35

September

Week 36

Week 37

Week 38

  • Filmed "Bricks of Knowledge" [http://youtube.com video on USER cloning] for KU team
  • Mailed Biobricks! (tat signal peptide, AMO, cytochromes)
  • Conducted Bioreactor experiment

Week 39