Team:Heidelberg/Templates/Del week15 EF

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08-08-2013

Amplification of FS_04 to FS_05; 6 kb

File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l.png
Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)
File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l cut.png
Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)
Reaction
what µl
D. acidovorans SPH-1 1
FS_04: (1/10) 4
FS_05: (1/10) 4
Phusion flash Master Mix 10
Conditions
Biometra TProfessional Basic
Cycles temperature [°C] Time [s]
1 98 10
30 98 1
58 5
72 1:30
1 72 7min
1 12 inf

Results:

  • Amplification of DelEF worked, though several side products and a smear occured
  • bands were cut out and DNA purified using QIAquick Gel Extraction Kit