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Team:Heidelberg/Templates/Del week10 OP
From 2013.igem.org
Revision as of 14:07, 30 September 2013 by Nils.kurzawa (Talk | contribs)
04-07-2013
Amplification from FS_12 to FS_13; 2.7 kb
- Reaction
| what | µl |
|---|---|
| D. acidovorans DSM-39 | 1 |
| FS_12: (1/10) | 1 |
| FS_13: (1/10) | 1 |
| Phusion Master Mix | 10 |
| DMSO | 1 |
| dd H2O | 6 |
- Conditions
| Biorad MyCycler | ||
|---|---|---|
| Cycles | temperature [°C] | Time [s] |
| 1 | 98 | 10 |
| 30 | 98 | 1 |
| 72 | 40 | |
| 1 | 72 | 420 |
| 1 | 4 | inf |
Results:
- Amplification of DelOP did not work
- Amplification will be repeated to exclude that pipetting errors were the reason for the failure
