Team:Heidelberg/Templates/Del week12 LP

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18-07-2013

Amplification from FS_13s to FS_15s; 6.4 kb

+=with DMSO, -=without DMSO; expected amplicon sizes: LP=6.4kbp, 8-9=3.1kbp, 21-09 and 20-09=8.1kbp, 22-13s=2.6kbp, AE=5.3kbp; run at 100 V, 0.8 % gel (TAE)

Amplifications of 18.07 and 19.07, cut

Reaction of DelLP

what	µl
D. acidovorans DSM-39	1
FS_13_short: (1/10)	2
FS_15_short: (1/10)	2
Phusion flash Master Mix	10
DMSO	1
dd H₂O	4

Conditions of Del LP

Biorad C1000 Touch Block A
Cycles	temperature [°C]	Time [s]
1	98	10
12	98	1
	65 ↓ 0.5	5
	72	2:10
18	98	1
	63	5
	72	2:10
1	72	10min
1	12	inf

Results:

The second try to amplify DelL-P did not work. There were no bands visible on the gel.
It does not make sense to try this again.