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Exeter/30 July 2013
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Miniprep, NanoDrop, SureClean of RBS + Cph8 (K592018)
NanoDrop + Sureclean data:
| Culture | Quickstart protocol (ng/µl) | Sureclean (ng/µl) |
|---|---|---|
| RBS + Cph8 | 17.5 | 34.6 |
| B0015 | 4.3 | 16.0 |
Liquid cultures
We made liquid cultures of:
Cph8 + RBS - K592018
B0015 - Terminator
K592022 - Cyan (w/RBS and promoter)
K864404 - Promoter, RBS and cyan
K592011 - Cyan pigment
S05058
Digest
We followed advice from a PhD student and did:
- Positive controls of:
- RFP cut with E and S
- RFP cut with X and P
- Negative control of:
- Just water
The buffer and BSA were vortexed before use to make sure they were completely defrosted.
We got fresh purite water each time we needed it.
We incubated at 37°C for 30 minutes and at 80°C for 20 minutes.
Take me back to the notebook.
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