Team:DTU-Denmark/Notebook/5 July 2013

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Contents

115

Main purpose

Prepare things for work in the lab at 115 which include autoclaving tubes and tips, making medium and plates with and without different antibiotics and make chemically competent cells.

Preparation of media for ATCC 19718 Nitrosomonas Europaea based on the protocol given.

Who was in the lab

Kristian, Kasia, Ariadni

Procedure

For chemically competent cells the protocol from iGEM [http://parts.igem.org/Help:Protocols/Competent_Cells Protocol for Competent cells] was used but we grew cells at 37°C.

Results

Competent cells need first to be tested.