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We ran an experiment proving the stabilization of a bacterial culture, the kinetic lasts for 12 hours, and the concentration of bacterial was stabilized for 5 consecutive hours.
In this experiment we succeed to stabilize the amount of living bacteria at a level of $0.015 OD_{600}$. Even better: the previsions gave us the right amount of bacteria.
The first part of the experiment followed a protocol close to the other:
1. A first period of 90 minutes in the dark for the bacteria to grow and produce KillerRed.
2. A long period of 4 hours at an illumination of maximal intensity. At this time, the effect of illumination were clear, and some parameters could have been adapted to fit these effects.
Then the model was used to predict which illumination could stabilized the population.
3. Following the model predictions, the illumination of the next period of timehas been at 70% of maximal intensity.
4. After 2 hours, as the fluorescence and the absorbance were slowly drifting away from the predictions, the illumination was put at 50% of maximal intensity for 2 hours, and then at 40% of maximal intensity.
| Time (min) | 0 | 90 | 330 | 450 | 570 | 720 |
|---|---|---|---|---|---|---|
| illumination (%) | 0 | 100 | 70 | 50 | 40 | end |
The curve of OD
The curve of OD
The curve of OD
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