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From 2013.igem.org

{ "date" : "2013-06-20", "author" : "emil-gabriele", "title" : "Linearization of psB1C3(Failed) and screening of SAM sinthethase", "content" : "We quantified yesterday inocula (1:1, 1:2, 1:4 of SAMsynthetase and R0010, both in pSB1C3).

Then, we screened the products of ligation (1:1, 1:2, 1:4 and R0010): digestion with EcoRI-HF and PstI-HF following the digestion protocol and agarose gel 1%.In these images we can only see the band of the backbone and no insert!", "tags" : "SAMsynthetase" }