Team:Paris Bettencourt/Notebook/Drug Screening/Thursday 3rd October.html

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Target

Race to Finish stuff

Plated new SirA, FprA, and FdxA transformations on CHL

Picked pSB1C3 CI and grew in LB broth

Z Score of BL21 dCysI pD002 pD003 with Gentamicin as positive hit control

Analytical Digestions of New Vectors (pAD1S, pAD2C, pAD3K, pAD4A)

Digest pSB1C3 CI with EcoRI and SpeI

Digest pSB3K3 with EcoRI and PstI

Digest pSB1A3 E0240 with XbaI and PstI

Ligation of CI promoter with GFP reporter and pSB3K3 backbone

Heat Shock Transformation of pSB3K3 CI GRP into NEBTurbo

PCR verify SirA, FprA, FdxA, and CI biobrick constructs