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From 2013.igem.org

{"date":"2013-07-01","author":"viola","title":"PCR of the double terminator BBa_B0015","content":"Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities:

	B0015 in pSB1AK3 219,4 ng/ µl	B0015 in pSB1C3 413,3 ng/ µl
H20	33.3 µl	33.3 µl
DNA	0.5 µl	0.5 µl
DNTPs	1 µl	1 µl
pref. FW	2.5 µl	2.5 µl
suff. RV	2.5 µl	2.5 µl
BUFFER	10 µl	10 µl
Phusion pol.	0.5 µl	0.5 µl

}}</html>\" I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html> on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.","tags":"EFE"}