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From 2013.igem.org

{"date" : "2013-07-25","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : " today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula!

As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!","tags" : " YF1_FixJ - FixK2"}