Team:DTU-Denmark/Notebook/10 July 2013

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Contents

208

Main purpose

Run gel with 9 PCR samples of Nir operon from Pseudomonas aeruginosa Transformation in e.coli

Who was in the lab

Ariadni,Henrike,Julia,Natalia

Procedure

Run gel

  • 6 samples from PCR of 09.07.2013
  • ladder
  • 3 samples from purifcation of 09.07.2013

Transformation in e.coli

According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes.

  • step 1:100 μl cells
  • step 2:1.5 μl of the resuspended DNA
  • step 6 : 90 sec (instead of 60 s)at 42 oC
  • step 9: incubation without shaking



Results

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