Team:Heidelberg/Templates/M-26-04-13

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Contents

Cloning Techniques/Strategies (Dominik)

  • BBa (BioBrick Standard)
  • Golden Gate
  • Gibson Assambly

Discussion on Experimental Procedures

  • Transformations
  • Cosmids
  • BACs

NRPS Library - with Discussion

  • main ideas
  • swaping of domains done already
  • Q: Biology behind the System? (short presentation by Ilia on monday?)

Agenda for next meeting (29.04.2013)

  • presentation of NRPS (library) (Ilia)
  • presentation about methodology (BAC, PAC, Cosmide, Gibson Assambly (Fanny, Flo, Hanna, Joshua, Nicos)
  • schedule (Hanna, Julia) ?

Ressource Collection

  • Vector Design
    • [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI]
    • [http://www.geneious.com/ Geneious]
  • Gibson Assembly
    • NEB Discripton & Kit
    • Reference:
  1. Gibson, D.G. et al. (2009) Nature Methods, 343–345.
  2. Gibson, D.G. et al. (2010) Nature Methods, 901–903.
  • Reserach on NRPS
    • [http://www.uni-marburg.de/fb15/ag-marahiel/research AG Marahiel, Marburg] --> Niels
    • [http://www.mpimf-heidelberg.mpg.de/gruppen/cytochrome AG Cryle, MPI Heidelberg] --> Konrad

further planning

  • Sat. 4. May: primer Design meeting ("Giant Inserts in E. coli")
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