Team:Tuebingen/Notebook/Protocols/inoue

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Chemo-competent E. coli cells

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Reagents

9.67 g	MnCl2 * 2 H2O
2.2 g	CaCl2 * 2 H2O
18.65 g	KCl
20.0 mL	PIPES (c = 0.5 M ; pH 6.7)
to 1 L	Aqua dest.

Procedure

Mix all reagents and fill up to 1 L with Aqua dest. Serilize through filtration (0.45 µm filter) and store at -20°C.

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