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Team:Heidelberg/Templates/MM week13p overview
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Revision as of 00:35, 4 October 2013 by TaniaChristiansen (Talk | contribs)
Goal
File:Heidelberg PIK1.svg
Vector map of the pIK1 plasmid.
File:Heidelberg PIK2.svg
Vector map of the pIK2 plasmid.
Back-up plan in case the genomic integration does not work. Two plasmids are to be constructed, pIK1 (containing BBa_I746200, pccB-accA, and sfp) and pIK2 (containing pccB-accA and sfp) in the pSB3C5 backbone. 6 fragments are to be amplified:
| fragment ID | source | primers | size [kb] |
|---|---|---|---|
| f1 | BBa_J04450 in pSB3C5 | IK32+IK33 | 3 |
| f2 | BBa_I746200 | IK26+IK27 | 2.3 |
| f3 | pccB-accA in pLF03 | IK38+IK29 | 3.4 |
| f4 | sfp in BAP1 | IK30+IK31 | 0.7 |
| f5 | BBa_J04450 in pSB3C5 | IK32+IK35 | 3 |
| f6 | pccB-accA in pLF03 | IK34+IK29 | 3.4 |
