Team:Heidelberg/Templates/MM week14p

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Revision as of 00:42, 4 October 2013 by Herbstk (Talk | contribs)

2013-07-29

1 µl of pLF03 miniPrep from 2013-06-04
  • make miniPrep -> very low yield (1-2 ng/µl)
  • load 1µl of 97ng/µl pLF03 miniPrep from 2013-06-04 on gel -> ca. 20 ng/µl
  • inoculate 50 ml LB+Amp with TOP10-pLF03, grow at 37°C

2013-07-30

1 µl of pLF03 midiPrep from 2013-07-30
  • make midiPrep -> 1352 ng/µl in 66.5 µl
  • medium was contaminated (also check with Fanny) -> low yields of previous miniPreps

2013-07-31

  • send 20 µl of 1:15 dilution of midiPrep to sequencing with primers IK07, IK36, IK37: File:Heidelberg PLF03 seq 2013-07-31.zip
  • perform multiple sequence alignment against assumed pLF03 sequence: IK07, IK36, IK37
  • deletion at the end of accA2 -> primer can not bind
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