Header

All the samples were sent for sequencing from their miniprep.

Begining of functionnal assays
INP_Streptavidin Alive, Dead and iGEM overnight inoculation (LB + chloramphenicol) and DH5alpha competent cells from glycerol stocks plates.

Sensing-Effector

PCR of the BBa_J23119 part from iGEM
-In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid.
The PCR of this promoter worked very well.