Team:Heidelberg/Templates/Del week18 overview

From 2013.igem.org

Revision as of 09:40, 4 October 2013 by Charly (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

SDS-PAGE

This week we carried out an SDS-PAGE with our clone D8w containing the pFSN plasmid. We used E.coli DH10ß as control. For our transformed E.coli a clear band at about 190 kDa was visible. This corresponds to the molecular weight of DelE (192,473 kDa). Consequently we showed that DelE is recombinantly expressed. Futhermore one could sense a very weak band at the height of about360 kDa. However this band could not be captured on an image. Consequently the samples have to be applied on a gel in a higher about again.

Retrieved from "http://2013.igem.org/Team:Heidelberg/Templates/Del_week18_overview"