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From 2013.igem.org

Contents 1 208 1.1 Main purpose 1.2 Who was in the lab 1.3 Procedure 1.3.1 Combine Nir operon with pZA21

208

Main purpose

• PCR with USER primers to combine Nir operon with pZA21

Who was in the lab

Ariadni,Henrike,Julia,Jakob

Procedure

Combine Nir operon with pZA21

Mastermix for 7 PCR tubes, Using the mastermix protocol we got from Mathilde.

triplets of each of the two Nir Operon extracts that we have made yesterday, including one negative control:

• Nir 2a(I,II,III)
• Nir 2b(I,II,III)

Using the following PCR program:

	time	Temp C
Denaturing	2 min	98
start of loop	10 sec	98
annealing	30 sec	60
elongation	9 min	72
goto start of loop 35 times
hold		10