Team:DTU-Denmark/Notebook/11 July 2013

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Contents

208

Main purpose

Who was in the lab

Ariadni,Henrike,Julia,Jakob

Procedure

Combine Nir operon with pZA21

Following Mathildes Protocol for DpnI digestion of our linear pZA21 w. Uracil incerts

Mastermix for 7 PCR tubes, Using the mastermix protocol we got from Mathilde.

triplets of each of the two Nir Operon extracts that we have made yesterday, including one negative control:

  • Nir 2a(I,II,III)
  • Nir 2b(I,II,III)

Using the following PCR program:

timeTemp C
Denaturing2 min98
start of loop 10 sec 98
annealing30 sec60
elongation9 min72
goto start of loop 35 times
hold 10