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From 2013.igem.org

Colony PCR

Colonies were picked with pipet tips and dipped into the PCR mixture.

Ingredient	amount [µl]
Overnight Culture	Top of a pipet tip
10x PCR buffer	2.0
MgCl2 (25mM)	2.0
Forward primer (10µM)	0.2
Reverse primer (10µM)	0.2
dNTPs (10mM)	1.0
Polymerase	0.5
Sterile water	ad 10.0
Total	10.0

The following PCR program was adjusted according to annealing conditions.

Cycles	temperature [°C]	Time [min:s]
1	98	2:00
35	98	0:05
	68 (annealing)	0:15
	72	1:00 per kbp
1	72	10:00
1	12	inf