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Team:NTNU-Trondheim/Notebook/June
From 2013.igem.org
Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts Promoter+RBS+RFP+term (BBa_I13521), Promoter+RBS+GFP+term (BBa_I13522),Promoter+RBS+CFP+term (BBa_I13600) and Pm+RBS+mCherry+term to competent ''E.coli'' DH5α cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)
For our transformation protocol, see the protocol page.
We transformed BioBricks consisting of fluorescent proteins. Transformed the parts YFP (BBa_E0030), CFP (BBa_ I13600), RFP (BBa_E1010), GFP (BBa_E0040), BFP (BBa_K592100) and SYFP (BBa_K864100).
We transferred mCherry from agar plate to liquid medium and incubated at 37° C.
We transferred the transformed cells from yesterday to liquid medium and incubated at 37°C ( protocol).
We isolated the DNA from the transformed mCherry cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.
| Sample | Concentration (ng/µl) |
|---|---|
| mCherry1 | 5,6 |
| mCherry2 | 7,3 |
We isolated the DNA from the transformed cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.
| Sample | Concentration (ng/µl) |
|---|---|
| CFP1 | 8,59 |
| CFP2 | 7,04 |
| BFP | 7,06 |
| SYFP | 5,42 |
| RFP1 | 5,04 |
| RFP2 | 7,52 |
| YFP | 6,79 |
| GFP | 5,27 |
This week we have established protocols for further work and designed primers for PCR.
