• Start
• Team
  • Members
  • TU Braunschweig
  • Official Team Profile
  • Attributions
• Project
  • Project Outline
  • Results
  • Engineering Principles
  • Approach
  • Potential Impact
  • Parts submitted to the registry
  • Notebook
  • Protocols
  • Acknowledgements
• Human Practices
  • Overview
  • Activities
  • Guide Part 1 - Communication strategy
  • Guide Part 2 - Implementation
  • Guide Part 3- Media Coverage Report
  • Guide Part 4 - Social Media Analysis
• Cooperations
• Safety
• Judging
• 

Results

In our project we were able to assemble two functional devices (BBa_K1073034 and BBa_K1073035) using standard Biobricks from the registry.
To establish a stable microbial consortium our engineered constructs consist of different operation units. We demonstrated the functionality of each unit by individual experiments:

1. Reporter-chromoproteins were successfully integrated into the final constructs

The included reporter-chromoproteins were visible to the naked eye in less than 24 h during incubation on agar plates or in liquid culture (fig. 1). The use of chromoproteins as reporter facilitated the distinction of the different bacterial strains we intended to cultivate in co-culture. Featured chromoproteins were amilGFP, eforRed and aeBlue which developed a yellowish, bright pink and dark blue color.

Fig. 1: Successful integration of reporter-chromoproteins into our constructs.
Liquid overnight cultures expressing the chromoproteins amilGFP (left), eforRed (middle), aeBlue (right).

However, eforRed and amilGFP can also be detected by fluorescence (fig. 2-4).

Fig. 2: Absorption and emission spectra of eforRed.

Fig. 3: Absorption and emission spectra of amilGFP.

Fig. 4: Bacteria expressing eforRed and amilGFP can clearly be distinguished in fluorescence microscopy.