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Exeter/4 July 2013
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Morning
- Tried to make 1 litre of clear agar using :
5g NaCl
15g Tryptone
15g Agar
5g Soytone
Tryptone, NaCl and soytone were added into a mixer with a magnetic stirrer. (was a yellow colour).
3g of agar were added to two 200ml durans. The Tryptone mix was then added to the durans, filling it up to 200ml.
Mixture was yellow, not colourless - the experiment was not successful. Durans put in the autoclave in the evening to let the agar set overnight.
Afternoon
- Transformation of competent cells
