Protocols

Here is a list of all the main protocols that we used for our constructions, you can download them as PDF files.

• Preparation of M9 medium
  
  
• TSS transformations
  
  
• pQE30 purification and analysis (Gel)
  
  
• Digestion of pQE30-alpha SNAP
  
  
• Digestion of BamHI-KillerRed-KpnI
  
  
• PCR of KillerRed with BamHI and KpnI restriction sites
  
  
• PCR of KillerRed-ssrA
  
  
• KillerRed kinetics