Team:DTU-Denmark/Notebook/15 July 2013

From 2013.igem.org

Revision as of 14:38, 15 July 2013 by Gosia (Talk | contribs)

Contents

208

Main purpose

  • Colony PCR of AMO, HAO, cytochromes and Nir transformants

Who was in the lab

Julia, Kristian, Gosia

Procedure

Colony PCR

Colony PCRwas performed according to standard method in order to check if transformed cells which grew on medium contain desired insert in pZA21 plasmid. Constructs for transformations contained one gene in plasmid pZA21 and were created using USER reaction. Inesrted genes are as follows: HAO, AMO, cycAX (cytochromes), Nir operon.

Several colonies were chosen from each plate to be checked. Names and numbers of colonies as well as plates names and primers are:

  • 1,2 - HAO in pZA21 USER, primers 18a, 18b
  • 3-10 - AMO in pZA21 USER, primers 17a, 17b
  • 11-24 - cycAX in pZA21 USER, primers 16a, 16b
  • 25-28 - Nir in pZA21 USER, primers 15a, 15b
  • RFP 1 - RFP 20 - RFP in pZA21, primers 14a, 14b
Retrieved from "http://2013.igem.org/Team:DTU-Denmark/Notebook/15_July_2013"