Team:Tuebingen/Project/Collaboration

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This year, we collaborated with Team BGU Israel who were working on a kill-switch mechanism. Team BGU Israel had sent us their pUC57 cI plasmid (BBa_K354000) that contains CRB resistance and a cI repressor gene with a Lac/Ara-1 IPTG inducible promoter. The promoter is induced by arabinose and IPTG, while glucose represses it. The cI protien is a repressor for our toxin system which is part of our self destruct mechanism. As long as there is cI in the cell, there is no expression of the toxins and therefore the bacteria still lives. As part of modelling the system, its is important to characterize the promoter regarding how IPTG, Arabinose and Gluose concetrations and the induction time have and impact on the amount of cI the cell produces.We had planned to characterize

Unfortunately, we were not able to complete the measurements with their parts because due to long delivery times their parts shipping arrived only shortly before Wiki Freeze.

Nevertheless we were able to help each other out with the planning and execution of the characterization of some parts. We thank the Team BGU for their efforts and the good conversations and hope that this collaboration will continue even more successfully next year