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Team:Groningen/SchematicOverview
From 2013.igem.org
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| The spider silk gene and the Pdes (heat sensing) promoter with CheY (movement) gene downstream of it are put in our backbone, and inserted into the Bacillus subtilis genome. |
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| The transformed B. subtilis is then put in a bath with a heated implant |
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| The secreted spider silk with strep-tag attaches to the implant its streptavidin coating. The coating is processed with evaporation, treated with kosmotropic ions and heated thoroughly |
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| The implant is now coated by the Coating GEMs and ready for use! |
