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From 2013.igem.org

Mirjam
Finally the colonies are growing on LB agar plates with ampicillin. The negative control does not show any colonies, the double negative does??, the positive control shows a lot of red colonies. On one of the four plates there are no colonies present, on two of them only a couple and on one a lot.

The colonies are picked and placed in LB medium with ampicillin to grow further for miniprep.

Inne
Did a PCR with 3 samples in duplo

Sample	Primer F	Primer R	Annealing temp.
1	without strep tag	without strep tag	78 C
2	with strep tag	without strep tag	78 C
3	without strep tag	with strep tag	80 C

Protocol:

amount	compound
28.2 uL	MilliQ
1.5 uL	5% DMSO
1 uL	each dNTP
10 uL	Phusion GC buffer
2.5 uL	Primer F
2.5 uL	Primer R
1 uL	Silk template BBa_16
0.3 uL	Phusion polymerase

Sander
did a Restriction digestion for Silk and Signal Sequences. incubation started at 11:00 for the signal sequences and 11:30 for the silk.