Team:Heidelberg/Templates/Indigoidine week8 overview

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Indigoidine Production - bpsA

We performed PCR screenings of the pMM64 (bpsA) and pMM65 (svp) plasmid for validation.

The PCR screenings showed that the pMM-plasmids are correct. We designed primers to assemble our first [http://parts.igem.org/Part:pSB1C3 pSB1C3] derived plasmid pKH1, which carries both the bpsA and the svp gene with the lacI-Promoter [http://parts.igem.org/Part:BBa_R0010?title=Part:BBa_R0010 BBa_R0010] and the ribosome binding site [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 BBa_B0034] before both the bpsA and the svp gene, respectively..

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