Team:Paris Bettencourt/Notebook/Phage Sensor/Friday 25th October.html

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Detect

Friday 25^th October

Colony PCR
Liquid Cloning

Liquid: NEB helper, parental pS002, parental pS006*, sSP008+pS002, sSP008+pS002’

Trafo: NEB helper+phageid-gRNA, parental ps006*+pS002’, pyrfF F+ pS002’+pS006*

conjugation of parental pS006* with pS009

Miniprep phagemid gRNA

Liquid library
Restreak for library

1) sSP008+pS002/pS002’ +Xgal
2) sSP008+pS002/pS002’ +Xgal + MMC
3) sSP008+pS002/pS002’ +MMC