Team:Groningen/Labwork/17 July 2013

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Revision as of 10:13, 17 July 2013 by Mirjam (Talk | contribs)

Mirjam
Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV.

A second confirmation of the constructs is done using PCR.

Inne
The E. coli cells with the BBa_k818000 that were to grow overnight didn't grow.
The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.


Autoclaved new tips (white yellow and blue) and PCR tubes.


Made 400 mL 0.8% Agarose solution.

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