Team:Shenzhen BGIC ATCG/parts
From 2013.igem.org
![Ball](https://static.igem.org/mediawiki/2013/a/ad/Eyeblack.png)
![Ball](https://static.igem.org/mediawiki/2013/4/4c/Eyewhite.png)
Playing with my eyes
aren't you?
Hi I am Dr. Mage!
A "budding" yeast cell!
Best BioBricks
Best BioBrick (Natural/Collection), BBa_K1051303, BBa_K1051300 ~ BBa_K1051306
K1051303
![](https://static.igem.org/mediawiki/2013/0/0c/K1051303.png)
K1051300
![](https://static.igem.org/mediawiki/2013/8/8d/K1051300.png)
K1051301
![](https://static.igem.org/mediawiki/2013/c/c0/K1051301.png)
K1051302
![](https://static.igem.org/mediawiki/2013/c/c6/K1051302.png)
K1051304
![](https://static.igem.org/mediawiki/2013/f/fa/K1051304.png)
K1051305
![](https://static.igem.org/mediawiki/2013/4/49/K1051305.png)
K1051306
![](https://static.igem.org/mediawiki/2013/5/5d/K1051306.png)
Best BioBrick (Engineered), BBa_K1051900
K1051900
![](https://static.igem.org/mediawiki/igem.org/c/ce/K1051900a.png)
![](https://static.igem.org/mediawiki/igem.org/f/fa/K1051900b.png)
Best Measurement, BBa_K1051257 ~ BBa_K1051259
K1051257
![](https://static.igem.org/mediawiki/2013/4/43/K1051257.png)
K1051258
![](https://static.igem.org/mediawiki/2013/d/d4/K1051258.png)
K1051259
![](https://static.igem.org/mediawiki/2013/e/ec/K1051259.png)
Devices List
Final Constructions
Final Constructions of E.coli
K1051910
![](https://static.igem.org/mediawiki/2013/d/d7/K1051910.png)
K1051911
![](https://static.igem.org/mediawiki/2013/0/0a/K1051911.png)
K1051912
![](https://static.igem.org/mediawiki/2013/b/bc/K1051912.png)
Final Constructions of Budding Yeast
K1051900a
![](https://static.igem.org/mediawiki/igem.org/c/ce/K1051900a.png)
K1051900b
![](https://static.igem.org/mediawiki/igem.org/f/fa/K1051900b.png)
K1051901
![](https://static.igem.org/mediawiki/2013/e/e3/K1051901.png)
K1051902
![](https://static.igem.org/mediawiki/2013/2/2c/K1051902.png)
K1051903
![](https://static.igem.org/mediawiki/2013/1/11/K1051903.png)
Promoter Verification
Verification of E.coli
K1051245
![](https://static.igem.org/mediawiki/2013/d/d8/K1051245.png)
K1051246
![](https://static.igem.org/mediawiki/2013/2/2b/K1051246.png)
K1051247
![](https://static.igem.org/mediawiki/2013/7/79/K1051247.png)
Verification of Budding Yeast
K1051307
![](https://static.igem.org/mediawiki/2013/c/c5/K1051307.png)
K1051355
![](https://static.igem.org/mediawiki/2013/1/12/K1051355.png)
K1051356
![](https://static.igem.org/mediawiki/2013/7/74/K1051356.png)
K1051357
![](https://static.igem.org/mediawiki/2013/4/48/K1051357.png)
K1051358
![](https://static.igem.org/mediawiki/2013/d/d6/K1051358.png)
K1051359
![](https://static.igem.org/mediawiki/2013/6/65/K1051359.png)
K1051365
![](https://static.igem.org/mediawiki/2013/4/40/K1051365.png)
K1051366
![](https://static.igem.org/mediawiki/2013/3/33/K1051366.png)
K1051367
![](https://static.igem.org/mediawiki/2013/4/4f/K1051367.png)
K1051368
![](https://static.igem.org/mediawiki/2013/8/8a/K1051368.png)
K1051369
![](https://static.igem.org/mediawiki/2013/5/52/K1051369.png)
Degradation Rate Measurement
Measurement of E.coli
K1051257
![](https://static.igem.org/mediawiki/2013/4/43/K1051257.png)
K1051258
![](https://static.igem.org/mediawiki/2013/d/d4/K1051258.png)
K1051259
![](https://static.igem.org/mediawiki/2013/e/ec/K1051259.png)
Measurement of Budding Yeast
K1051278
![](https://static.igem.org/mediawiki/2013/b/b2/K1051278.png)
K1051279
![](https://static.igem.org/mediawiki/2013/1/1d/K1051279.png)
K1051280
![](https://static.igem.org/mediawiki/2013/1/1a/K1051280.png)
K1051281
![](https://static.igem.org/mediawiki/2013/5/5e/K1051281.png)
K1051288
![](https://static.igem.org/mediawiki/2013/8/88/K1051288.png)
Targeting Peptides Verification
K1051150
![](https://static.igem.org/mediawiki/2013/d/db/K1051150.png)
K1051151
![](https://static.igem.org/mediawiki/2013/9/97/K1051151.png)
K1051152
![](https://static.igem.org/mediawiki/2013/0/0e/K1051152.png)
K1051153
![](https://static.igem.org/mediawiki/2013/c/cb/K1051153.png)
CRISPRi Verification
K1051852
![](https://static.igem.org/mediawiki/2013/3/33/K1051852.png)
Alternative Splicing Verification
K1051706
![](https://static.igem.org/mediawiki/2013/2/2b/K1051706.png)
Basic Parts
Universal Parts
Reporters
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051000 | Reporter | Stop codon free RFP in RFC[23] standard | Xiang LI | 675 |
BBa_K1051001 | Reporter | Stop codon free ECFP in RFC[23] standard | Xiang LI | 716 |
BBa_K1051002 | Reporter | Stop codon free EYFP in RFC[23] standard | Xiang LI | 717 |
BBa_K1051003 | Reporter | Stop codon free GFP in RFC[23] standard | Xiang LI | 714 |
BBa_K1051004 | Reporter | Stop codon free mOrange in RFC[23] standard | Xiang LI | 738 |
Yeast Terminators
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051006 | Terminator | Saccharomycescerevisiae TBY-1 terminator with stopcodon | Xiang LI | 31 |
BBa_K1051007 | Terminator | S. cerevisiae SSV7 terminator with stopcodon | Xiang LI | 39 |
BBa_K1051008 | Terminator | S. cerevisiae CAA terminator with stopcodon | Xiang LI | 18 |
E. coli Terminators
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051009 | Terminator | e.coli terminator ilvGEDA_T with stop codon | Xiang LI | 92 |
Cyclin Promoters
Yeast
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051300 | Regulatory | cln2 promoter (G1 phase to S phase) | Kequan Lin | 1000 |
BBa_K1051301 | Regulatory | clb2 promoter (during G2 phase) | Kequan Lin | 1063 |
BBa_K1051302 | Regulatory | clb5 promoter (during G1 phase) | Kequan Lin | 648 |
BBa_K1051304 | Regulatory | cln3 promoter (M phase to G1 phase) | Kequan Lin | 1075 |
BBa_K1051305 | Regulatory | met16 promoter (M phase to G1 phase) | Zhu Shuang | 551 |
BBa_K1051306 | Regulatory | met28 promoter (S phase) | Zhu Shuang | 500 |
E. coli
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051400 | Regulatory | promoter->dnaAp1 | Bingwei Zheng | 359 |
BBa_K1051401 | Regulatory | promoter->dnaAp1 no RBS | Bingwei Zheng | 184 |
BBa_K1051402 | Regulatory | promoter->gyrB | Bingwei Zheng | 188 |
BBa_K1051403 | Regulatory | promoter->gyrB no RBS | Bingwei Zheng | 158 |
BBa_K1051404 | Regulatory | promoter->ftsQp | Bingwei Zheng | 485 |
BBa_K1051405 | Regulatory | promoter->ftsQp no RBS | Bingwei Zheng | 198 |
Degradation Tags
Yeast
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051200 | DNA | ubiquitin | wanling ZHOU | 1146 |
BBa_K1051201 | DNA | D-BOX | wanling ZHOU | 39 |
BBa_K1051202 | DNA | cln1-PEST | wanling ZHOU | 489 |
BBa_K1051203 | DNA | Cln2-PEST | wanling ZHOU | 537 |
BBa_K1051204 | DNA | cln3-PEST | wanling ZHOU | 588 |
E. coli
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051206 | Tag | The degradation tag in E. coil ,M0050 with TAATAA. | Rui Guan | 39 |
BBa_K1051207 | Tag | The degradation tag in E. coil ,M0051 with TAATAA. | Rui Guan | 45 |
BBa_K1051208 | Tag | The degradation tag in E. coil ,M0052 with TAATAA. | Rui Guan | 39 |
Targeting Peptides
Mitochondria
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051100 | Coding | targeting peptide to mitochondria inner membrane | Jinchun Zhang | 209 |
BBa_K1051101 | Coding | Cox4 targeting peptide to mitochondria inner membrane | Jinchun Zhang | 75 |
BBa_K1051102 | Coding | targeting peptide to mitochondria inner membrane | Jinchun Zhang | 141 |
BBa_K1051103 | Coding | TOM22, targeting peptide to mitochondria outer membrane | Jinchun Zhang | 69 |
BBa_K1051104 | Coding | VDAC1;targeting peptide to mitochondria outer membrane | Jinchun Zhang | 93 |
BBa_K1051105 | Coding | TOM40;targeting peptide to mitochondria outer membrane | Jinchun Zhang | 147 |
BBa_K1051106 | Coding | targeting peptide to mitochondria outer membrane | Jinchun Zhang | 96 |
BBa_K1051107 | Coding | Tom70;targeting peptide to mitochondria outer membrane | Jinchun Zhang | 90 |
BBa_K1051108 | Coding | targeting peptide to mitochondria outer membrane;Tom20 | Jinchun Zhang | 120 |
BBa_K1051109 | Coding | targeting peptide to mitochondria matrix;MIA40 | Jinchun Zhang | 93 |
Centrosome
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051110 | Coding | ScSfi1;targeting peptide to centrosome | Jinchun Zhang | 63 |
BBa_K1051111 | Coding | ScSfi1E; targeting peptide to centrosome; | Jinchun Zhang | 63 |
BBa_K1051112 | Coding | ScKar1;targeting peptide to centrosome | Jinchun Zhang | 60 |
BBa_K1051113 | Coding | ScSac3;targeting peptide to centrosome | Jinchun Zhang | 57 |
Nucleus
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051114 | Coding | H2A2;targeting peptide to nucleus | Jinchun Zhang | 408 |
Vacuolar
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051115 | Coding | ZRC1;Vacuolar Membrane Targeting Protein | Jinchun Zhang | 396 |
Actin
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051116 | Coding | Actin Targeting Protein | Jinchun Zhang | 1779 |
Plasma Membrane
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051117 | Coding | Plasma Membrane Targeting Sequence | Jinchun Zhang | 12 |
Peroxisomes
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051118 | Coding | ScPEX21p;baking yeast targeting peptide to peroxisome ; | Jinchun Zhang | 30 |
CRISPRi Verification
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051800 | Coding | protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon. | Shihong Chen | 4164 |
BBa_K1051801 | Device | Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib | Shihong Chen | 388 |
BBa_K1051801 | Device | Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib | Shihong Chen | 388 |
Alternative Splicing Verification
Name | Type | Description | Designer | Length |
---|---|---|---|---|
BBa_K1051700 | DNA | SRC1 Intron -4bp at 5' | Yang Zhou | 129 |
BBa_K1051701 | DNA | SRC1 Intron+GG | Yang Zhou | 132 |
BBa_K1051702 | DNA | SRC1 Intron+CGG | Yang Zhou | 133 |
BBa_K1051703 | DNA | SRC1 Intron with additional 6bp at both directions | Yang Zhou | 142 |
Parts Sequencing
All of our parts have been sequenced to get verification.
Sequencing example of K1051701
![](https://static.igem.org/mediawiki/2013/8/8d/Sequence_Result.png)
Figure. Sequencing Result
Alignment example of K1051701
![](https://static.igem.org/mediawiki/2013/7/74/Alignment.png)
Figure. Alignment Result