Team:Groningen/Labwork/18 July 2013

From 2013.igem.org

Sander
prepared 20 tubes for use in -80 c Glyserol stock

Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).
Afterwards concentration of DNA was determined via nanodrop.

Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL

During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1).